Recombinant SARS-CoV-2 Nucleocapsid antibody

Catalog No. ABIN6953120

This anti-SARS-CoV-2 Nucleocapsid antibody is a Human Monoclonal antibody detecting SARS-CoV-2 Nucleocapsid in ELISA, IF and Neut. Suitable for SARS Coronavirus-2 (SARS-CoV-2) and SARS Coronavirus (SARS-CoV). This Primary Antibody has been cited in 1 publication.

Quick Overview for Recombinant SARS-CoV-2 Nucleocapsid antibody (ABIN6953120)

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Antibody Type: Recombinant Antibody

Reactivity: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV)

Host: Human

Clonality: Monoclonal

Conjugate: This SARS-CoV-2 Nucleocapsid antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Neutralization (Neut)

Clone: CR3009 (03-009)

Product Details anti-SARS-CoV-2 Nucleocapsid Antibody

Specificity: This antibody recognizes and binds the non-linear/conformational epitope of the N protein of SARS CoV and also binds the SARS CoV2 nucleoprotein.

Characteristics: Recombinant monoclonal antibody to Covid-19 & SARS-CoV Nucleoprotein.

Several anti-SARS-CoV-2 Nucleoprotein antibodies based on clone CR3009 are available:
Human IgG1, IgG2, IgG3, IgA and IgM for use in neutralization assays and as serological controls

• ABIN6953060 - Human IgG1
• ABIN6953114 - Human IgG2
• ABIN6953120 - Human IgG3
• ABIN6953115 - Human IgA
• ABIN6953061 - Human IgM

Rabbit IgG, mouse IgG2b and mouse IgM for detection applications, co-labelling studies and animal model research

• ABIN6953062 - Rabbit IgG
• ABIN6953112 - Mouse IgG2b
• ABIN6953111 - Mouse IgM

Human and mouse Fab and Fab2 formats with His-tags, for applications where antibody fragments are desirable and for site-specific functionalization

• ABIN6953116 - Human Fab fragment, His tag
• ABIN6953118 - Human F(ab)2 fragment, His tag
• ABIN6953117 - Mouse Fab fragment, His tag
• ABIN6953119 - Mouse F(ab)2 fragment, His tag

Human IgG1 and mouse IgG2b Fc-Mutant formats, which are aimed at discerning Fc-dependent and Fc-independent effector functions and facilitating research into the role of antibody-dependent enhancement (ADE)

• ABIN6953110 - Human IgG1, Fc-Mutant
• AABIN6953113 - Mouse IgG2b, Fc-Mutant

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by cloning the variable regions of the scFvs selected from phage display libraries into separate vectors for IgG1 heavy-chain and light-chain expression. The harvested supernatents were then purified on protein A columns.

Isotype: IgG3

Application Details

Application Notes: This antibody is recommended for detection of SARS CoV2 protein N (nucleoprotein). This antibody binds both the nucleocapsid protein of the SARS-CoV and SARS CoV-2 (2019-nCoV). Initial characterization of the antibody for binding to 2019-nCoV was done using ELISA. This antibody shows potential to be used for development of diagnostic assays. Various isotype versions of the antibody namely human IgG1, IgG3, IgM, IgA and the less common IgG2 and IgG4 are available for the investigation of their role in response to SARS CoV2. Competitive ELISA of this antibody with CR3009 suggests that both these antibodies bind different epitopes of the N protein of SARS CoV. Thus, a combination of these two antibodies is suggested for virus capture assays. Immunofluorescence staining was used to demonstrate binding of CR3018 to SARS-CoV infected Vero cells.

Competitive ELISA of both anti-nucleocapsid antibodies suggests that they bind different, non-overlapping epitopes of the N protein of SARS-CoV. Thus, a combination of these two antibodies is suggested for virus capture assays. Clone CR3018 binds the amino acid residues between 11-19 of the N protein of SARS-CoV, while clone CR3009 binds a non-linear/conformational epitope of the N protein of SARS-CoV, both of which are sufficiently conserved to permit binding of these antibodies to SARS-CoV-2.

Comment:

This reformatted human antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

References for anti-SARS-CoV-2 Nucleocapsid antibody (ABIN6953120)

van den Brink, Ter Meulen, Cox, Jongeneelen, Thijsse, Throsby, Marissen, Rood, Bakker, Gelderblom, Martina, Osterhaus, Preiser, Doerr, de Kruif, Goudsmit: "Molecular and biological characterization of human monoclonal antibodies binding to the spike and nucleocapsid proteins of severe acute respiratory syndrome coronavirus." in: Journal of virology, Vol. 79, Issue 3, pp. 1635-44, (2005) (PubMed).

Target Details for SARS-CoV-2 Nucleocapsid

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Alternative Name: SARS-CoV-SARS-CoV-2 Nucleocapsid Protein2 N

Target Type: Viral Protein

Background: NP, NC, Protein N, Nucleocapsid protein, SARS-CoV Protein N, SARS-CoV Nucleocapsid protein, SARS Coronavirus, SARS-CoV-2, SARS CoV 2, 2019-nCoV

UniProt: P0DTC9